IFRA Analytical Method for Parabens in Fragrance Raw Materials Using LC-MS/MS

The document outlines an analytical protocol developed by the International Fragrance Association (IFRA) for detecting and quantifying nine parabens in fragrance raw materials using LC-MS/MS. The method employs multiple reaction monitoring (MRM) with two transitions per paraben for identification, and quantification is achieved through internal standard calibration. The analysis has a quantification range of 0.5 to 100 mg/kg, with a 20x dilution of samples. It details the preparation of mobile phases, standards, and internal standard solutions, as well as sample preparation procedures. The protocol also provides MS/MS parameters, calibration methods, and the required equipment for the analysis, including retention times for each compound. This method ensures accurate detection and quantification of parabens in fragrance products.